English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background   Plasmodium vivax  is one of the widespread human malarial parasites accounting for 75% of malaria epidemics. However, there is no baseline information about the status and nature of genetic variation of  Plasmodium  species circulating in various parts of Pakistan. The present study was aimed at observing the molecular epidemiology and genetic variation of  Plasmodium vivax by  analysing its merozoite surface protein-3α ( msp-3α ) and merozoite surface protein-3β ( msp-3β ) genes, by using suballele, species-specific, combined nested PCR/RFLP detection techniques.    Methods  A total of 230 blood samples from suspected subjects tested slide positive for vivax malaria were collected from Punjab, Sindh, Khyber Pakhtunkhwa, and Balochistan during the period May 2012 to December 2013. Combined nested PCR/RFLP technique was conducted using  Pvmsp-3α  and  Pvmsp-3β  genetic markers to detect extent of genetic variation in clinical isolates of  P. vivax  in the studied areas of Pakistan.    Results  By PCR,  P. vivax,  202/230 (87.82%), was found to be widely distributed in the studied areas. PCR/RFLP analysis showed a high range of allelic variations for both  msp-3α  and  msp-3β  genetic markers of  P. vivax,  i.e., 21 alleles for  msp-3α  and 19 for  msp-3β . Statistically a significant difference (p ≤ 0.05) was observed in the genetic diversity of the suballelic variants of  msp-3α  and  msp-3β  genes of  P. vivax.Conclusion  It is concluded that  P. vivax  populations are highly polymorphic and diverse allelic variants of  Pvmsp-3α  and  Pvmsp-3β  are present in Pakistan.

PCR/RFLP-Based Analysis of Genetically Distinct Plasmodium vivax Population of Pvmsp-3α and Pvmsp-3β genes in Pakistan