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Background  Gametocytes, the blood stages responsible for  Plasmodium falciparum  transmission, contain electron dense organelles, traditionally named osmiophilic bodies, that are believed to be involved in gamete egress from the host cell. In order to provide novel tools in the cellular and molecular studies of osmiophilic body biology, a  P. falciparum  transgenic line in which these organelles are specifically marked by a reporter protein was produced and characterized.    Methodology  A  P. falciparum  transgenic line expressing an 80-residue N-terminal fragment of the osmiophilic body protein Pfg377 fused to the reporter protein DsRed, under the control of  pfg377  upstream and downstream regulatory regions, was produced.    Results  The transgenic fusion protein is expressed at the appropriate time and stage of sexual differentiation and is trafficked to osmiophilic bodies as the endogenous Pfg377 protein. These results indicate that a relatively small N-terminal portion of Pfg377 is sufficient to target the DsRed reporter to the gametocyte osmiophilic bodies.    Conclusions  This is the first identification of a  P. falciparum  aminoacid sequence able to mediate trafficking to such organelles. To fluorescently tag such poorly characterized organelles opens novel avenues in cellular and imaging studies on their biogenesis and on their role in gamete egress.

Specific tagging of the egress-related osmiophilic bodies in the gametocytes of Plasmodium falciparum