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Fermentation Expression of F1 by E. coli MC1060 (pBRK-F1) was studied in a 4-liter computer-controlled fermentor using a rich medium devoid of animal products: soy protein extract, trace elements and glycerol as carbon source (SY). This medium enabled efficient bacterial growth to a high cell density (60 OD600, Fig 1A). No loss of the expression plasmid was observed under these conditions. Unexpectedly, high and constant level of F1 expression was observed throughout the fermentation (up to 24 hours) even at 28°C (no temperature shift to 37°C was required, Fig. 1B). Moreover, at a high cell density (>10 OD600), F1 was released from the encapsulated E. coli cells and accumulated up to 0.9 g/L in the culture medium (Fig. 1C).

microbial cell factories

Production and purification of high molecular weight oligomers of <it>Yersinia pestis </it>F1 capsular antigen released by high cell density culture of recombinant <it>Escherichia coli </it>cells carrying the <it>caf1 </it>operon

Production and purification of high molecular weight oligomers of Yersinia pestis F1 capsular antigen released by high cell density culture of recombinant Escherichia coli cells carrying the caf1 operon