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Background Bacteriophage infections cause serious problems in both research laboratories and large biotechnological companies. Once infected by bacteriophages, bacterial cultures are usually completely destroyed, as phage lytic development in a bacterium ends up with cell lysis and liberation of progeny phages that infect neighbor bacterial cells. A possibility of spreading of bacteriophages throughout a laboratory is even more dangerous than a loss of a single culture. Namely, subsequent cultures may be infected, which can lead to cultivation problems lasting even several months or longer. Therefore, a method for inhibition of bacteriophage lytic development in infected cultures would be useful. Perhaps it is not difficult in small cultures (e.g. flask cultures), when simple sterilization of the whole material and a flask should be sufficient. However, phage contamination in bioreactors is a serious technical problem indeed.

microbial cell factories

A simple emergency procedure to be used if biotechnological protein production is endangered by bacteriophage infection of <it>Escherichia coli </it>cultures: effective inhibition of bacteriophage lytic development in infected cultures by removing a carbon source from the medium

A simple emergency procedure to be used if biotechnological protein production is endangered by bacteriophage infection of Escherichia coli cultures: effective inhibition of bacteriophage lytic development in infected cultures by removing a carbon source from the medium