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Background Human collagen prolyl 4-hydroxylase (C-P4H), an ER lumenal protein, is a key enzyme in the biosynthesis of collagens and consists of two different subunits forming an α2β2 tetramer. Heterologous cytoplasmic production of an active C-P4H in Escherichia coli using a bicistronic vector with the T5-lac and tet promoters and the strain OrigamiTM as a host was described earlier [1]. Gene optimisation of the β subunit that is identical to protein disulfide isomerase and selection of the best induction conditions improved the obtained activity of recombinant C-P4H in shake flask cultivations further by a factor of 50 [2].

microbial cell factories

Use of the <it>tetA</it>-promoter in fed-batch cultivations: Repeated supply of anhydrotetracycline is necessary for production of tetrameric collagen prolyl 4-hydroxylase in <it>Escherichia coli</it>

Use of the tetA-promoter in fed-batch cultivations: Repeated supply of anhydrotetracycline is necessary for production of tetrameric collagen prolyl 4-hydroxylase in Escherichia coli