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Background Chinese Hamster Ovary (CHO) cell is one of the major cell lines used for complex recombinant protein production. During CHO cell culture, loss in viability attributed to apoptosis often results in lower recombinant protein yield and affects protein quality. Through the individual targeting of four apoptosis genes identified via expression profiling studies, four apoptosis resistant CHO GT (Gene Targeted) cell lines were constructed. These cell lines enabled prolonged culture viability, higher maximum viable cell densities and significant increase in recombinant human interferon gamma (IFN-γ) yields. Furthermore, it was observed that the IFN-γ from CHO GT cells has a higher level of sialic acid content.

Impact of apoptosis gene targeting on recombinant protein glycosylation