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Background  Protein production as secretory-form is a powerful tool in industrial enzyme production due to the simple purification procedure.  Streptomyces lividans  is a versatile host for secretory production of useful proteins. In order to expand the amount of secreted protein, signal peptide sequences, which encourage protein secretion from inside cell to extracellular environment, are one of the most significant factors. In this study, we focused on  Streptomyces lividans  as a host strain to secrete useful proteins, and screened for signal peptides from the biomass-degradation enzymes derived from  Thermobifida fusca  YX and  S. lividans .    Results  Three candidate signal peptides were isolated and evaluated for their protein secretion ability using β-glucosidase derived from  T. fusca  YX, which is a non-secreted protein, as a model protein. Using  S. lividans  xylanase C signal peptide, the amount of produced the β-glucosidase reached 10 times as much as that when using  Streptomyces cinnamoneus  phospholipase D signal peptide, which was identified as a versatile signal peptide in our previous report. In addition, the introduction of the β-glucosidase fused to xylanase C signal peptide using two kinds of plasmid, pUC702 and pTYM18, led to further protein secretion, and the maximal level of produced the β-glucosidase increased up to 17 times (1.1 g/l) compared to using only pUC702 carrying the β-glucosidase fused to  S. cinnamoneus  phospholipase D signal peptide.    Conclusion  In the present study, we focused on signal peptide sequences derived from biomass degradation enzymes, which are usually secreted into the culture supernatant, and screened for signal peptides leading to effective protein secretion. Using the signal peptides, the hyper-protein secretion system was successfully demonstrated for the cytoplasmic β-glucosidase.

Hyper secretion of Thermobifida fusca β-glucosidase via a Tat-dependent signal peptide using Streptomyces lividans