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Background  Different parts of  Podocarpus henkelii  have been used in many cultures around the world to treat ailments such as cholera, stomach diseases, rheumatism, cancer, canine distemper in dogs and gall sickness in cattle. The aim of this study was to evaluate the biological activity and toxicity of isolated compounds from  Podocarpus henkelii  after an earlier study indicated a promising activity in crude extracts against viral pathogens of veterinary importance.    Methods  The antibacterial and antifungal activity of two biflavonoids 7, 4’, 7”, 4”’-tetramethoxy amentoflavone (TMA), isoginkgetin (IGG) and podocarpus flavone–A (PFA) isolated from the leaves of  Podocarpus henkelii  were determined using a serial microplate dilution method with tetrazolium violet as growth indicator. The cytotoxicity of compounds TMA and IGG were determined on different cell types using a tetrazolium-based colorimetric cellular assay (MTT). The Ames test was used to determine their mutagenic activities.    Results  TMA had reasonable antifungal activity against  Aspergillus fumigatus  (MIC = 30 μg/ml). IGG had a wide spectrum of activity against four bacterial and two fungal pathogens with much higher selectivity index values obtained for  A. fumigatus  and  Cryptococcus neoformans  (SI > 30). PFA had a broad spectrum of activity against  Enterococcus faecalis  and  Pseudomonas aeruginosa  (SI > 15) and less activity against the two fungal pathogens. In both the cytotoxicity assays and Ames mutagenicity test using  Salmonella typhimurium  strains TA98 and TA100, TMA and IGG had no deleterious effect on the different cell types and did not induce mutations in the Ames test.    Conclusion  Although the antimicrobial activities of the isolated compounds were not that exciting, the compounds had no cytotoxic activity at the highest concentration (1000 μg/ml) tested against all three cell lines. IGG was the most active against  E. coli ,  S. aureus ,  A. fumigatus  and  C. neoformans , exhibiting both antibacterial and antifungal activity with good selectivity index values. PFA had a broad spectrum of activity against  E. faecalis  and  P. aeruginosa.  The two compounds isolated had low toxicity and no genotoxic activity in the Ames test.

Antimicrobial activity, toxicity and selectivity index of two biflavonoids and a flavone isolated from Podocarpus henkelii (Podocarpaceae) leaves