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Background  Immunomodulators are substances that modify immune system response to a threat. Immunomodulators modulate and potentiate the immune system, keeping it highly prepared for any threat. The immunomodulatory effect of the traditional medicine  Tinospora crispa  is investigated in this work.    Methods   T. crispa  ethanol extract was fractionated by using different solvents. The ethanol extract and effective isolated fraction were used to investigate the potential immunomodulatory effect of different  T. crispa  doses ranging from 25 μg/mL to 1000 μg/mL on RAW 246.7 cells by detecting intracellular INF-γ, IL-6, and IL-8 expressions. The antioxidant activity of  T. crispa  was evaluated through FRAP and DPPH. The total phenolic and total flavonoid contents were also quantified.    Results  Results show that  T. crispa  extract has higher antioxidant potential than ascorbic acid. The FRAP value of  T. crispa  extract is 11011.11 ± 1145.42 μmol Fe +2 /g, and its DPPH inhibition percentage is 55.79 ± 7.9, with 22 μg/mL IC50. The results also reveal that the total phenolic content of  T. crispa  extract is 213.16- ± 1.31 mg GAE/g dry stem weight, and the total flavonoid content is 62.07- ± 39.76 mg QE/g dry stem weight.  T. crispa  crude extract and its isolated fraction significantly stimulate RAW264.7 cell viability ( P ≤  0.05) and intracellular INF-γ, IL-6, and IL-8 expressions. The results of LC-MS show that four of the active compounds detected in the  T. crispa  isolated fraction are cordioside, quercetin, eicosenoic acid (paullinic acid), and boldine.    Conclusions  The results of this study obviously indicate that  T. crispa  has immunomodulatory effects through the stimulation of INF-γ, IL-6, and IL-8 expressions. LC-MS phytochemical analysis showed that the  T. crispa  fraction has cordioside, quercetin, eicosenoic acid (paullinic acid), and boldine, which may be responsible for the immunostimulator effect of  T. crispa .

Immunomodulatory effect of an isolated fraction from Tinospora crispa on intracellular expression of INF-γ, IL-6 and IL-8