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Background  The aim of this study was to evaluate the antimicrobial activity and the cytotoxicity of the ethanol crude extract, fractions and isolated compounds from the twigs of  Eriosema robustum , a plant used for the treatment of coughs and skin diseases.    Methods  Column chromatographic and spectroscopic techniques were used to isolate and identify eight compounds, robusflavones A ( 1 ) and B ( 2 ), orostachyscerebroside A ( 3 ), stigmasterol ( 4 ), 1- O -heptatriacontanoyl glycerol ( 5 ), eicosanoic acid ( 6 ), 3- O -β-D-glucopyranoside of sitosterol ( 7 ) and 6-prenylpinocembrin ( 8 ), from  E. robustum . A two-fold serial microdilution method was used to determine the minimum inhibitory concentration (MIC) against fungi and bacteria, and the 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide reduction assay was used to evaluate the cytotoxicity.    Results  Fraction B had significant antimicrobial activity against  Aspergillus fumigatus  and  Cryptoccocus neoformans  (MIC 0.08 mg/ml), whilst the crude extract and fraction A had moderate activity against  A. fumigatus  and  Candida albicans  (MIC 0.16 mg/ml). Fraction A however had excellent activity against  Staphylococcus aureus  (MIC 0.02 mg/ml),  Enterococcus faecalis  and  Escherichia coli  (MIC 0.04 mg/ml). The crude extract had significant activity against  S. aureus ,  E. faecalis  and  E. coli . Fraction B had good activity against  E. faecalis  and  E. coli  (MIC 0.08 mg/ml). All the isolated compounds had a relatively weak antimicrobial activity. An MIC of 65 μg/ml was obtained with robusflavones A ( 1 ) and B ( 2 ) against  C. albicans  and  A. fumigatus , orostachyscerebroside A ( 3 ) against  A. fumigatus , and robusflavone B ( 2 ) against  C. neoformans . Compound  8  had the best activity against bacteria (average MIC 55 μg/ml). The 3 fractions and isolated compounds had LC 50  values between 13.20 to > 100 μg/ml against Vero cells yielding selectivity indices between 0.01 and 1.58.    Conclusion  The isolated compounds generally had a much lower activity than expected based on the activity of the fractions from which they were isolated. This may be the result of synergism between different compounds in the complex extracts or fractions. The results support the traditional use of  E. robustum  to treat infections. The crude extract had a good activity and low preparation cost, and may be useful in topical applications to combat microbial infections.

Antimicrobial activity and cytotoxicity of the ethanol extract, fractions and eight compounds isolated from Eriosema robustum (Fabaceae)