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Methods We performed BV pharmacopuncture therapy with different concentrations on neuropathic pain in a rat model on the 7th day after ligating the L5 nerve as suggested by Kim and Chung. Rats were divided into a control group (treated with normal saline), experimental group I (treated with constantly increased BV concentration, from 1.67×10mg/kg to 8.35×10mg/kg, total 3.22×10mg), and experimental group II (treated fixed high concentration, 3.58×10mg/kg, total 3.22×10mg). BV pharmacopuncture was injected to Huantiao (GB30) every other day for 18 days. To identify any therapeutic effect, foot withdrawal threshold to mechanical and thermal stimulation, nerve conduction velocity (NCV), and c-Fos immunological reactivity in the dorsal horn of the spinal cord were analyzed.

P01.06. The effect of bee venom pharmacopuncture therapy in a neuropathic pain rat model