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Abstract Background The rapid expansion in the availability of genome and DNA sequence information has opened up new possibilities for the development of methods for detecting free-living protozoa in environmental samples. The protozoan <it>Blepharisma japonicum </it>was used to investigate a rapid and simple detection system based on polymerase chain reaction amplification (PCR) from organisms immobilised on FTA paper. Results Using primers designed from the α-tubulin genes of <it>Blepharisma</it>, specific and sensitive detection to the equivalent of a single <it>Blepharisma </it>cell could be achieved. Similar detection levels were found using water samples, containing <it>Blepharisma</it>, which were dried onto Whatman FTA paper. Conclusion This system has potential as a sensitive convenient detection system for <it>Blepharisma </it>and could be applied to other protozoan organisms.</p

A rapid and simple method of detection of Blepharisma japonicum using PCR and immobilisation on FTA paper.