English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Abstract Background <it>Agrobacterium vitis</it> is a causal agent of crown-gall disease. Trifolitoxin (TFX) is a peptide antibiotic active only against members of a specific group of α-proteobacteria that includes <it>Agrobacterium</it> and its close relatives. The ability of TFX production by an avirulent strain of <it>Agrobacterium</it> to reduce crown gall disease is examined here. Results TFX was shown to be inhibitory <it>in vitro</it> against several <it>A. vitis</it> strains. TFX production, expressed from the stable plasmid pT2TFXK, conferred biological control activity to an avirulent strain of <it>A. vitis.</it> F2/5, against three virulent, TFX-sensitive strains of <it>A. vitis</it> tested on <it>Nicotiana glauca.</it> F2/5(pT2TFXK) is significantly reduces number and size of galls when co-inoculated with tumorigenic strain CG78 at a 10:1 ratio, but is ineffective at 1:1 or 1:10 ratios. F2/5(pT2TFXK) is effective when co-inoculated with tumorigenic strain CG435 at 10:1 and 1:1 ratios, but not at a 1:10 ratio. When F2/5(pT2TFXK) is co-inoculated with CG49 at a 10:1 ratio, the incidence of gall formation does not decline but gall size decreases by more than 70%. A 24 h pre-inoculation with F2/5(pT2TFXK) does not improve biological control at the 1:10 ratio. Conclusions TFX production by an avirulent strain of <it>Agrobacterium</it> does confer in that strain the ability to control crown gall disease on <it>Nicotiana glauca.</it> This is the first demonstration that the production of a ribosomally synthesized, post-translationally modified peptide antibiotic can confer reduction in plant disease incidence from a bacterial pathogen.</p

bmc biotechnology

Expression of a crown gall biological control phenotype in an avirulent strain of Agrobacterium vitis by addition of the trifolitoxin production and resistance genes.