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Background  To date, over 150 genetically modified (GM) crops are widely cultivated. To comply with regulations developed for genetically modified organisms (GMOs), including labeling policies, many detection methods for GMO identification and quantification have been developed.    Results  To detect the entrance and exit of unauthorized GM crop events in China, we developed a novel quadruplex real-time PCR method for simultaneous detection and quantification of GM cotton events GHB119 and T304-40 in cotton-derived products (based on the 5′-flanking sequence) and the insect-resistance gene  Cry2Ae . The limit of detection was 10 copies for GHB119 and  Cry2Ae  and 25 copies for T304-40. The limit of quantification was 25 copies for GHB119 and  Cry2Ae  and 50 copies for T304-40. Moreover, low bias and acceptable standard deviation and relative standard deviation values were obtained in quantification analysis of six blind samples containing different GHB119 and T304-40 ingredients.    Conclusions  The developed quadruplex quantitative method could be used for quantitative detection of two GM cotton events (GHB119 and T304-40) and  Cry2Ae  gene ingredient in cotton derived products.

A novel quadruplex real-time PCR method for simultaneous detection of Cry2Aeand two genetically modified cotton events (GHB119 and T304-40)