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Background  The filamentous fungus  Trichoderma reesei  ( Hypocrea jecorina)  is an important source of cellulases for use in the textile and alternative fuel industries. To fully understand the regulation of cellulase production in  T. reesei , the role of a gene known to be involved in carbon regulation in  Aspergillus nidulans , but unstudied in  T. reesei , was investigated.    Results  The  T. reesei  orthologue of the  A. nidulans creB  gene, designated  cre2 , was identified and shown to be functional through heterologous complementation of a  creB  mutation in  A. nidulans . A  T. reesei  strain was constructed using gene disruption techniques that contained a disrupted  cre2  gene. This strain, JKTR2-6, exhibited phenotypes similar to the  A. nidulans creB  mutant strain both in carbon catabolite repressing, and in carbon catabolite derepressing conditions. Importantly, the disruption also led to elevated cellulase levels.    Conclusions  These results demonstrate that  cre2  is involved in cellulase expression. Since the disruption of  cre2  increases the amount of cellulase activity, without severe morphological affects, targeting  creB  orthologues for disruption in other industrially useful filamentous fungi, such as  Aspergillus oryzae ,  Trichoderma harzianum  or  Aspergillus niger  may also lead to elevated hydrolytic enzyme activity in these species.

Disruption of Trichoderma reesei cre2, encoding an ubiquitin C-terminal hydrolase, results in increased cellulase activity