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Background  MicroRNA-34 is a family of three miRNAs that have been reported to function as tumor suppressor miRNAs and show decreased expression in various cancers. Here, we examine functions of miR-34c in basal-like breast cancer cells.    Methods  Data from The Cancer Genome Atlas (TCGA) were used for evaluation of expression in primary breast cancers. Cellular processes affected by miR-34c were investigated by thymidine incorporation, Annexin V-assays and cell cycle analysis using breast cancer cell lines. Effects on potential targets were analyzed with qPCR and Western blot.    Results  TCGA data revealed that miR-34c was expressed at lower levels in basal-like breast cancer tumors and low expression was associated with poor prognosis. Ectopic expression of miR-34c in basal-like breast cancer cell lines resulted in suppressed proliferation and increased cell death. Additionally, miR-34c influenced the cell cycle mainly by inducing an arrest in the G2/M phase. We found that expression levels of the known cell cycle-regulating miR-34 targets  CCND1 ,  CDK4  and  CDK6 , were downregulated upon miR-34c expression in breast cancer cell lines. In addition, the levels of  CDC23 , an important mediator in mitotic progression, were suppressed following miR-34c expression, and siRNAs targeting  CDC23  mimicked the effect of miR-34c on G2/M arrest. However, protein levels of  PRKCA , a predicted miR-34c target and a known regulator of breast cancer cell proliferation were not influenced by miR-34c.    Conclusions  Together, our results support the role of miR-34c as a tumor suppressor miRNA also in breast cancer.    Electronic supplementary material  The online version of this article (doi:10.1186/1471-2407-14-538) contains supplementary material, which is available to authorized users.

Expression of miR-34c induces G2/M cell cycle arrest in breast cancer cells