English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background   miR-182  is one of the most significantly up-regulated miRNAs in hepatocellular carcinoma (HCC). Metastasis suppressor 1 ( MTSS1 ), one target gene of  miR-182 , plays an important role in the metastasis of cancers. However, it remains unclear what role does function and mechanism of  miR-182  and  MTSS1 play in HCC.    Methods   miR-182  expression was tested in 86 cases of paired HCC and normal tissues by real-time PCR and the relationships between  miR-182  expression and clinicopathological parameters were analyzed. The expression of  MTSS1  was evaluated by immunohistochemistry and western blot in the above tissues and its correlation with  miR-182  expression was analyzed. Moreover, western blot and invasion assays were performed after transfection of  pre - miR-182  or  anti - miR-182  to HCC cell lines. In addition, luciferase assays was performed to confirm the regulation of  miR-182  on  MTSS1 .    Results  Compared with normal tissue,  miR-182  was up-regulated and  MTSS1  was down-regulated in HCC tissues. Moreover, the over-expression of  miR-182  was correlated with intrahepatic metastasis ( p  = 0.034) and poor prognosis ( p  = 0.039) of HCC patients. There was a negative correlation between  miR-182  and  MTSS1  expression in both HCC tissues ( r  = −0.673,  p  < 0.01) and HCC cell lines ( r  = −0.931,  p  = 0.021). Furthermore, the up-regulation of  miR-182  resulted in the down-regulation of  MTSS1  and increased invasive potential of HUH-1, and reverse results were also confirmed when the expression of  miR-182  was inhibited. In addition, the results of the luciferase assay demonstrated the targeted regulation of  miR-182  on  MTSS1 .    Conclusions   miR-182  could promote metastasis of HCC and inhibit the expression of  MTSS1 .  miR-182  and  MTSS1  are potential prognostic markers and/or therapeutic targets in HCC.

MicroRNA-182 downregulates metastasis suppressor 1 and contributes to metastasis of hepatocellular carcinoma