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Background  Ficolin-2 coded by  FCN2  gene is a soluble serum protein and an innate immune recognition element of the complement system.  FCN2  gene polymorphisms reveal distinct geographical patterns and are documented to alter serum ficolin levels and modulate disease susceptibility.    Methods  We employed a real-time PCR based on Fluorescence Resonance Energy Transfer (FRET) method to genotype four functional SNPs including  -986 G > A  (#rs3124952),  -602 G > A  (#rs3124953),  -4A > G  (#rs17514136) and  +6424 G > T  (#rs7851696) in the ficolin-2 ( FCN2)  gene. We characterized the  FCN2  variants in individuals representing Brazilian (n = 176), Nigerian (n = 180), Vietnamese (n = 172) and European Caucasian ethnicity (n = 165).    Results  We observed that the genotype distribution of three functional SNP variants (− 986 G > A, -602 G > A  and  -4A > G ) differ significantly between the populations investigated ( p  < 0.0001). The SNP variants were highly linked to each other and revealed significant population patterns. Also the distribution of haplotypes revealed distinct geographical patterns ( p  < 0.0001).    Conclusions  The observed distribution of the  FCN2  functional SNP variants may likely contribute to altered serum ficolin levels and this may depend on the different disease settings in world populations. To conclude, the use of FRET based real-time PCR especially for  FCN2  gene will benefit a larger scientific community who extensively depend on rapid, reliable method for  FCN2  genotyping.

Reliable and rapid characterization of functional FCN2 gene variants reveals diverse geographical patterns