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Background  Antigen specificity and IgG subclass could be significant in the natural history of Chagas' disease. The relationship between the different stages of human Chagas' disease and the profiles of total IgG and its subclasses were thus analysed here; they were directed against a crude  T. cruzi  extract and three recombinant antigens: the  T. cruzi  kinetoplastid membrane protein-11 (rKMP-11), an internal fragment of the  T. cruzi  HSP-70 protein 192-433 , and the entire  Trypanosoma rangeli  HSP-70 protein.    Methods  Seventeen Brazilian acute chagasic patients, 50 Colombian chronic chagasic patients (21 indeterminate and 29 cardiopathic patients) and 30 healthy individuals were included. Total IgG and its subtypes directed against the above-mentioned recombinant antigens were determined by ELISA tests.    Results  The  T. cruzi  KMP-11 and  T. rangeli  HSP-70 recombinant proteins were able to distinguish both acute from chronic chagasic patients and infected people from healthy individuals. Specific antibodies to  T. cruzi  crude antigen in acute patients came from IgG3 and IgG4 subclasses whereas IgG1 and IgG3 were the prevalent isotypes in indeterminate and chronic chagasic patients. By contrast, the specific prominent antibodies in all disease stages against  T. cruzi  KMP-11 and  T. rangeli  HSP-70 recombinant antigens were the IgG1 subclass.    Conclusion   T. cruzi  KMP-11 and the  T. rangeli  HSP-70 recombinant proteins may be explored together in the immunodiagnosis of Chagas' disease.  Polarising the IgG1 subclass of the IgG response to  T. cruzi  KMP-11 and  T. rangeli  HSP-70 recombinant proteins could have important biological effects, taking into account that this is a complement fixing antibody.

Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patients