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Results A calibration curve produced by 10-fold dilutions of HIV virion in HIV negative saliva collected in the OM-505 was linear (R=0.9951) from 57,273 to 621 HIV copies/mL. In clinical isolates, PVL averaged 431,865 HIV copies/mL (range: 62 to 7,604,620 HIV copies/mL) whilst SVL averaged 23,267 HIV copies/mL (range: 153 to 220,104 HIV copies/mL). SVL was not detected in 15 samples and could not be determined in 5 samples due to viscosity and cellular debris causing problems during extraction. In 12/17 patients SVL was lower than PVL. Conclusion HIV, if present, can be detected accurately in saliva down to 621 HIV copies/mL. SVL does not correlate with PVL and thus cannot be used to accurately determine HIV carriage, but in most cases HIV shedding is low or nonexistent.

bmc infectious diseases

Comparison of plasma and salivary HIV loads determined via a coupling of the Abbott HIV detection system with the DNA Genotek OMNIgene™ DISCOVER (OM-505) kits