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Method Fifty isolates of MRSA from different specimens like blood, urine, exudates and respiratory were collected from 2010 to 2011. MRSA screen slide latex agglutination test kit (Denka Seikan, Japan) was used for PBP2′ detection and Oxacillin Resistant Screen Agar Base (ORSAB) with supplement (Oxoid Ltd., UK) was used for detection of MRSA from the isolates with ATCC controls. The detection of mecA gene in the MRSA isolates was considered as the reference method for determining the sensitivity of each phenotype rapid method studied.

bmc infectious diseases

Comparison of rapid methods PBP2′ detection, ORSAB and mecA for detection of methicillin- resistant Staphylococcus aureus (MRSA) in a tertiary care centre, Chennai, India