English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background   In vitro  studies show that  Leishmania  infection decreases the adhesion of inflammatory phagocytes to connective tissue by a mechanism dependent on the modulation of integrin function. However, we know little about the influence of this reduction in leukocyte adhesion on parasite dissemination from the infection site.    Methods  In this work, we used a model of chronic peritonitis induced by thioglycollate to study the effect of  L. amazonensis  infection on the ability of inflammatory phagocyte populations to migrate from an inflammatory site to the draining lymph node. Uninfected or  Leishmania -infected thioglycollate-elicited peritoneal exudate cells were transferred from C57BL/6 to BALB/c mice or from Ly5.1 +  to Ly5.1 -  mice. The transferred cells were injected into the peritoneal cavity and tracked to the draining lymph node.    Results  Migrating cells corresponded to approximately 1% of the injected leukocytes. The proportion of migrating CD11b + CD11c +  (myeloid dendritic cell) was lower after incubation with  Leishmania  (1.3 to 2.6 times lower in the experiments using C57BL/6 to BALB/c animals and 2.7 to 3.4 times lower in the experiments using Ly5.1 +  to Ly5.1 -  animals) than after leukocyte incubation with medium alone (P < 0.01). There was no consistent decrease in the migration of CD11b + F4/80 +  (macrophage) or SSC hi  GR-1 +  (neutrophil) populations.    Conclusions  Coincubation with  Leishmania  changes the migratory pattern of dendritic cells  in vivo . Such changes in dendritic cell migration may be associated with immunological events that maintain inflammation at the sites of infection.    Electronic supplementary material  The online version of this article (doi:10.1186/1471-2334-14-450) contains supplementary material, which is available to authorized users.

Leishmania amazonensis infection impairs dendritic cell migration from the inflammatory site to the draining lymph node