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Background Pseudomonas is one of the predominant microorganisms of chronic lung infections. Bacteria growing in biofilm often develop multicellular, three-dimensional structures known as micro colonies. Pseudomonas colonizes the lungs by forming biofilm micro colonies throughout the lung. In this study, using Pseudomonas spp. artificial biofilm was grown on flow cell chamber and images were taken using Confocal Laser Scanning Microscope (CLSM), to establish an experimental model for artificial biofilm in vitro.

A confocal microscopic study on biofilm formed by Pseudomonas spp. isolated from lower respiratory tract infection from HIV and non-HIV populations