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Background Effective health care delivery to the majority of perinatally exposed infants is hampered by lack of access to accurate HIV diagnosis in infancy. Polymerase chain reaction is the most sensitive test to diagnose HIV-1 infection in children born to HIV seropositive mothers. The purpose of this study was to assess the feasibility and accuracy of using dried blood spot (DBS) technology in performing HIV-1 DNA PCR using Roche Amplicor HIV-1 DNA PCR version 1.5 for diagnosis in children less than 18 months of age.

Evaluation of HIV-1 polymerase chain reaction (PCR) technique using dried blood spots (DBS) for diagnosis of perinatal transmission of HIV