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Background The nematode Caenorhabditis elegans is one of the popular model hosts for the study of the evolutionarily conserved mechanism of microbial pathogenesis and innate immunity. C. elegans can be effectively used to study the dynamics of polymicrobial infections. Proteus mirabilis, an opportunistic pathogen, does not cause death in C. elegans. In this study the C. elegans was pre-infected with Staphylococcus aureus to make the C. elegans immunocompromised to study the effect of P. mirabilis in the host.

Response of Caenorhabditis elegans during subsequent infections with Gram positive and Gram negative bacteria