English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background  Anisakiasis is an important fish-borne zoonosis provoked by larval stages of nematodes belonging to the genus  Anisakis . The detection and identification of human infections is difficult. This is due to: a) the low specificity of the clinical features and symptomatology related to human infections; b) the paucity of diagnostic features of larvae found in granulomatous lesions characteristic of "invasive anisakiasis"; and c) the lack morphological characters diagnostic at the specific level when larvae of  Anisakis  are detected. Thus, molecular-based diagnostic approaches are warranted.    Method  We have developed a PCR method that amplifies the DNA of  Anisakis  spp. in fixed paraffin-embedded tissues. This method was applied to a granuloma removed from a human case of intestinal anisakiasis in Italy. Specific primers of the mtDNA  cox2  gene were used and sequence analysis was performed according to the procedures already established for species of  Anisakis .    Results  The sequence obtained (629 bp) was compared with those of the other species of  Anisakis  which have so far been genetically characterized and with sequences obtained from larval stages of  Anisakis  collected from the Mediterranean fish  Engraulis encrasicolus . This enabled the genetic identification of the larva in the human tissue as  A. pegreffii . This is the first instance of human intestinal anisakiasis diagnosed using PCR of DNA purified from a fixed eosinophilic granuloma embedded in paraffin.    Conclusion  The case of human anisakiasis presented reinforces the pathological significance of the species  A. pegreffii  to humans. The molecular/genetic methodological approach based on mtDNA  cox2  sequence analysis, described here, can allow easy and rapid identification of  Anisakis  spp. in formalin-fixed and paraffin embedded tissues removed from cases of either gastric or intestinal human anisakiasis.

bmc infectious diseases

First molecular identification of the zoonotic parasite Anisakis pegreffii(Nematoda: Anisakidae) in a paraffin-embedded granuloma taken from a case of human intestinal anisakiasis in Italy