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These, after having been separated, centrifugated and implanted on an adapted medium which consists of modified three-dimensional jaluronic acid, develop in vitro an extended layer of tissue (dimensions 100–200 cmq), which then will be grafted separately (after 4 weeks from the biopsy) on the surface of the ulcer. For the correct execution of such procedure are necessary four surgical steps, in sequence:

The biotechnologies in the treatment of neuro-vascular ulcers in the elder. Cultivated autografts of skin: fibroblasts and/or cheratinocytes