English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Previous studies indicated that the micro integral membrane protein located within the media place of the integral membrane protein of  Lactobacillus plantarum  CGMCC 1258 had protective effects against the intestinal epithelial injury. In our study, we mean to establish micro integral membrane protein -knockout  Lactobacillus plantarum  (LPKM) to investigate the change of its protective effects and verify the role of micro integral membrane protein on protection of normal intestinal barrier function.    Methods  Binding assay and intestinal permeability were performed to verify the protective effects of micro integral membrane protein on intestinal permeability  in vitro  and  in vivo . Molecular mechanism was also determined as the zonulin pathway. Clinical data were also collected for further verification of relationship between zonulin level and postoperative septicemia.    Results  LPKM got decreased inhibition of EPEC adhesion to NCM460 cells. LPKM had lower ability to alleviate the decrease of intestinal permeability induced by enteropathogenic-e.coli, and prevent enteropathogenic-e.coli -induced increase of zonulin expression. Overexpression of zonulin lowered the intestinal permeability regulated by  Lactobacillus plantarum . There was a positive correlation between zonulin level and postoperative septicemia. Therefore, micro integral membrane protein could be necessary for the protective effects of  Lactobacillus plantarum  on intestinal barrier.    Conclusion  MIMP might be a positive factor for  Lactobacillus plantarum  to protect the intestinal epithelial cells from injury, which could be related to the zonulin pathway.

bmc gastroenterology

Knockout of MIMP protein in lactobacillus plantarumlost its regulation of intestinal permeability on NCM460 epithelial cells through the zonulin pathway