English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  In plants, eIF4E translation initiation factors and their eIFiso4E isoforms are essential susceptibility factors for many RNA viruses, including potyviruses. Mutations altering these factors are a major source of resistance to the viruses. The  eIF4E  allelic series is associated with specific resistance spectra in crops such as  Capsicum annum . Genetic evidence shows that potyviruses have a specific requirement for a given 4E isoform that depends on the host plant. For example,  Tobacco etch virus  (TEV) uses eIF4E1 to infect  Capsicum annuum  but uses eIFiso4E to infect  Arabidopsis thaliana . Here, we investigated how TEV exploits different translation initiation factor isoforms to infect these two plant species.    Results  A complementation system was set up in  Arabidopsis  to test the restoration of systemic infection by TEV. Using this system,  Arabidopsis  susceptibility to TEV was complemented with a susceptible pepper  eIF4E1  allele but not with a resistant allele. Therefore, in  Arabidopsis , TEV can use the pepper eIF4E1 instead of the endogenous eIFiso4E isoform so is able to switch between translation initiation factor 4E isoform to infect the same host. Moreover, we show that overexpressing the pepper  eIF4E1  alleles is sufficient to make  Arabidopsis  susceptible to an otherwise incompatible TEV strain. Lastly, we show that the resistant  eIF4E1  allele is similarly overcome by a resistance-breaking TEV strain as in pepper, confirming that this  Arabidopsis  TEV-susceptibility complementation system is allele-specific.    Conclusion  We report here a complementation system in  Arabidopsis  that makes it possible to assess the role of pepper  pvr2-eIF4E  alleles in susceptibility to TEV. Heterologous complementation experiments showed that the idiosyncratic properties of the 4E and iso4E proteins create a major checkpoint for viral infection of different hosts. This system could be used to screen natural or induced eIF4E alleles to find and study alleles of interest for plant breeding.

Specific requirement for translation initiation factor 4E or its isoform drives plant host susceptibility to Tobacco etch virus