English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  The pepper fruit is the second most consumed vegetable worldwide. However, low temperature affects the vegetative development and reproduction of the pepper, resulting in economic losses. To identify cold-related genes regulated by abscisic acid (ABA) in pepper seedlings, cDNA representational difference analysis was previously performed using a suppression subtractive hybridization method. One of the genes cloned from the subtraction was homologous to  Solanum tuberosum MBF1  ( StMBF1 ) encoding the coactivator multiprotein bridging factor 1. Here, we have characterized this  StMBF1  homolog (named  CaMBF1 ) from  Capsicum annuum  and investigated its role in abiotic stress tolerance.    Results  Tissue expression profile analysis using quantitative RT-PCR showed that  CaMBF1  was expressed in all tested tissues, and high-level expression was detected in the flowers and seeds. The expression of  CaMBF1  in pepper seedlings was dramatically suppressed by exogenously supplied salicylic acid, high salt, osmotic and heavy metal stresses. Constitutive overexpression of  CaMBF1  in  Arabidopsis  aggravated the visible symptoms of leaf damage and the electrolyte leakage of cell damage caused by cold stress in seedlings. Furthermore, the expression of  RD29A ,  ERD15 ,  KIN1 , and  RD22  in the transgenic plants was lower than that in the wild-type plants. On the other hand, seed germination, cotyledon greening and lateral root formation were more severely influenced by salt stress in transgenic lines compared with wild-type plants, indicating that  CaMBF1 -overexpressing  Arabidopsis  plants were hypersensitive to salt stress.    Conclusions  Overexpression of  CaMBF1  in  Arabidopsis  displayed reduced tolerance to cold and high salt stress during seed germination and post-germination stages.  CaMBF1  transgenic  Arabidopsis  may reduce stress tolerance by downregulating stress-responsive genes to aggravate the leaf damage caused by cold stress.  CaMBF1  may be useful for genetic engineering of novel pepper cultivars in the future.

Reduced tolerance to abiotic stress in transgenic Arabidopsis overexpressing a Capsicum annuummultiprotein bridging factor 1