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Background  The  opaque2  mutant is valuable for producing maize varieties with enhanced nutritional value. However, the exact mechanisms by which it improves protein quality and creates a soft endosperm texture are unclear. Given the importance of improving nutritional quality in grain crops, a better understanding of the physiological basis for these traits is necessary.    Results  In this study, we combined transcript profiling and proteomic analysis to better understand which genes and proteins are altered by  opaque2  in the W64A inbred line. These analyses showed that the accumulation of some lysine-rich proteins, such as sorbitol dehydrogenase and glyceraldehyde3-phosphate dehydrogenase, was increased in mature kernels and may contribute substantially to the lysine content of  opaque2  endosperm. Some defense proteins such as beta-glucosidase aggregating factor were strongly down regulated and may be regulated directly by  opaque2 . The mutant also had altered expression of a number of starch biosynthesis genes and this was associated with a more highly crystalline starch.    Conclusions  The results of these studies revealed specific target genes that can be investigated to further improve nutritional quality and agronomic performance of high lysine maize lines, particularly those based on the presence of the  opaque2  mutation. Alteration of amylopectin branching patterns in  opaque2  starch could contribute to generation of the soft, starchy endosperm.

bmc plant biology

Identification and characterization of lysine-rich proteins and starch biosynthesis genes in the opaque2mutant by transcriptional and proteomic analysis