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Background   TIFY  is a large plant-specific transcription factor gene family. A subgroup of  TIFY  genes named JAZ (Jasmonate-ZIM domain) has been identified as repressors of jasmonate (JA)-regulated transcription in  Arabidopsis  and other plants. JA signaling is involved in many aspects of plant growth/development and in defense responses to biotic and abiotic stresses. Here, we identified the TIFY genes (designated  PvTIFY ) from the legume common bean ( Phaseolus vulgaris)  and functionally characterized  PvTIFY10C  as a transcriptional regulator.    Results  Nineteen genes from the  PvTIFY  gene family were identified through whole-genome sequence analysis. Most of these were induced upon methyl-JA elicitation. We selected  PvTIFY10C  as a representative JA-responsive  PvTIFY  gene for further functional analysis. Transcriptome analysis via microarray hybridization using the newly designed Bean Custom Array 90 K was performed on transgenic roots of composite plants with modulated (RNAi-silencing or over-expression)  PvTIFY10C  gene expression. Data were interpreted using Gene Ontology and MapMan adapted to common bean. Microarray differential gene expression data were validated by real-time qRT-PCR expression analysis. Comparative global gene expression analysis revealed opposite regulatory changes in processes such as RNA and protein regulation, stress responses and metabolism in  PvTIFY10C  silenced vs. over-expressing roots. These data point to transcript reprogramming (mainly repression) orchestrated by  PvTIFY10C . In addition, we found that several  PvTIFY  genes, as well as genes from the JA biosynthetic pathway, responded to P-deficiency. Relevant P-responsive genes that participate in carbon metabolic pathways, cell wall synthesis, lipid metabolism, transport, DNA, RNA and protein regulation, and signaling were oppositely-regulated in control vs.  PvTIFY10C -silenced roots of composite plants under P-stress. These data indicate that  PvTIFY10C  regulates, directly or indirectly, the expression of some P-responsive genes; this process could be mediated by JA-signaling.    Conclusion  Our work contributes to the functional characterization of  PvTIFY  transcriptional regulators in common bean, an agronomically important legume. Members from the large  PvTIFY  gene family are important global transcriptional regulators that could participate as repressors in the JA signaling pathway. In addition, we propose that the JA-signaling pathway involving  PvTIFY  genes might play a role in regulating the plant response/adaptation to P-starvation.

Common bean (Phaseolus vulgarisL.) PvTIFY orchestrates global changes in transcript profile response to jasmonate and phosphorus deficiency