English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Cotton ( Gossypium  spp.) is widely cultivated due to the important economic value of its fiber. However, extreme environmental degradation impedes cotton growth and production. Receptor-like kinase (RLK) proteins play important roles in signal transduction and participate in a diverse range of processes in response to plant hormones and environmental cues. Here, we introduced an RLK gene ( GbRLK ) from cotton into  Arabidopsis  and investigated its role in imparting abiotic stress tolerance.    Results   GbRLK  transcription was induced by exogenously supplied abscisic acid (ABA), salicylic acid, methyl jasmonate, mock drought conditions and high salinity. We cloned the promoter sequence of this gene  via  self-formed adaptor PCR. Sequence analysis revealed that the promoter region contains many  cis -acting stress-responsive elements such as ABRE, W-Box, MYB-core, W-Box core, TCA-element and others. We constructed a vector containing a 1,890-bp sequence in the 5′ region upstream of the initiation codon of this promoter and transformed it into  Arabidopsis thaliana . GUS histochemical staining analysis showed that  GbRLK  was expressed mainly in leaf veins, petioles and roots of transgenic  Arabidopsis , but not in the cotyledons or root hairs.  GbRLK  promoter activity was induced by ABA, PEG, NaCl and  Verticillium dahliae.  Transgenic  Arabidopsis  with constitutive overexpression of  GbRLK  exhibited a reduced rate of water loss in leaves  in vitro , along with improved salinity and drought tolerance and increased sensitivity to ABA compared with non-transgenic Col-0  Arabidopsis . Expression analysis of stress-responsive genes in  GbRLK Arabidopsis  revealed that there was increased expression of genes involved in the ABA-dependent signaling pathway ( AtRD20, AtRD22  and  AtRD26 ) and antioxidant genes ( AtCAT1, AtCCS, AtCSD2  and  AtCSD1 ) but not ion transporter genes ( AtNHX1, AtSOS1 ).    Conclusions   GbRLK  is involved in the drought and high salinity stresses pathway by activating or participating in the ABA signaling pathway. Overexpression of  GbRLK  may improve stress tolerance by regulating stress-responsive genes to reduce water loss.  GbRLK  may be employed in the genetic engineering of novel cotton cultivars in the future. Further studying of  GbRLK  will help elucidate abiotic stress signaling pathways.

A receptor-like kinase gene (GbRLK) from Gossypium barbadense enhances salinity and drought-stress tolerance in Arabidopsis