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Background  While the genetic transformation of the major cereal crops has become relatively routine, to date only a few reports were published on transgenic triticale, and robust data on T-DNA integration and segregation have not been available in this species.    Results  Here, we present a comprehensive analysis of stable transgenic winter triticale cv. Bogo carrying the selectable marker gene  HYGROMYCIN PHOSPHOTRANSFERASE  ( HPT ) and a synthetic  green fluorescent protein  gene ( gfp) . Progeny of four independent transgenic plants were comprehensively investigated with regard to the number of integrated T-DNA copies, the number of plant genomic integration loci, the integrity and functionality of individual T-DNA copies, as well as the segregation of transgenes in T 1  and T 2  generations, which also enabled us to identify homozygous transgenic lines. The truncation of some integrated T-DNAs at their left end along with the occurrence of independent segregation of multiple T-DNAs unintendedly resulted in a single-copy segregant that is selectable marker-free and homozygous for the  gfp  gene. The heritable expression of  gfp  driven by the maize  UBI-1  promoter was demonstrated by confocal laser scanning microscopy.    Conclusions  The used transformation method is a valuable tool for the genetic engineering of triticale. Here we show that comprehensive molecular analyses are required for the correct interpretation of phenotypic data collected from the transgenic plants.

Analysis of T-DNA integration and generative segregation in transgenic winter triticale (x Triticosecale Wittmack)