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Background  Mitogen activated protein kinase (MAPK) cascade is an important signaling cascade that operates in stress signal transduction in plants. The biologically active monoterpenoid indole alkaloids (MIA) produced in  Catharanthus roseus  are known to be induced under several abiotic stress conditions such as wounding, UV-B etc. However involvement of any signaling component in the accumulation of MIAs remains poorly investigated so far. Here we report isolation of a novel abiotic stress inducible  Catharanthus roseus  MAPK,  CrMPK3  that may have role in accumulation of MIAs in response to abiotic stress.    Results  CrMPK3 expressed in bacterial system is an active kinase as it showed auto-phosphorylation and phosphorylation of Myelin Basic Protein. CrMPK3 though localized in cytoplasm, moves to nucleus upon wounding. Wounding, UV treatment and MeJA application on  C. roseus  leaves resulted in the transcript accumulation of  CrMPK3  as well as activation of MAPK in  C. roseus  leaves. Immuno-precipitation followed by immunoblot analysis revealed that wounding, UV treatment and methyl jasmonate (MeJA) activate CrMPK3 .  Transient over-expression of  CrMPK3  in  C. roseus  leaf tissue showed enhanced expression of key MIA biosynthesis pathway genes and also accumulation of specific MIAs.    Conclusion  Results from our study suggest a possible involvement of  CrMPK3  in abiotic stress signal transduction towards regulation of transcripts of key MIA biosynthetic pathway genes, regulators and accumulation of major MIAs.

bmc plant biology

CrMPK3, a mitogen activated protein kinase from Catharanthus roseusand its possible role in stress induced biosynthesis of monoterpenoid indole alkaloids