English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Treatment of plants with HrpN Ea , a protein of harpin group produced by Gram-negative plant pathogenic bacteria, induces plant resistance to insect herbivores, including the green peach aphid  Myzus persicae , a generalist phloem-feeding insect. Under attacks by phloem-feeding insects, plants defend themselves using the phloem-based defense mechanism, which is supposed to involve the phloem protein 2 (PP2), one of the most abundant proteins in the phloem sap. The purpose of this study was to obtain genetic evidence for the function of the  Arabidopsis thaliana  (Arabidopsis) PP2-encoding gene  AtPP2-A1  in resistance to  M. persicae  when the plant was treated with HrpN Ea  and after the plant was transformed with  AtPP2-A1 .    Results  The electrical penetration graph technique was used to visualize the phloem-feeding activities of apterous agamic  M. persicae  females on leaves of Arabidopsis plants treated with HrpN Ea  and an inactive protein control, respectively. A repression of phloem feeding was induced by HrpN Ea  in wild-type (WT) Arabidopsis but not in  atpp2-a1 /E/142, the plant mutant that had a defect in the  AtPP2-A1  gene, the most HrpN Ea -responsive of 30  AtPP2  genes. In WT rather than  atpp2-a1 /E/142, the deterrent effect of HrpN Ea  treatment on the phloem-feeding activity accompanied an enhancement of  AtPP2-A1  expression. In PP2OETAt ( AtPP2-A1 -overexpression transgenic  Arabidopsis thaliana ) plants, abundant amounts of the  AtPP2-A1  gene transcript were detected in different organs, including leaves, stems, calyces, and petals. All these organs had a deterrent effect on the phloem-feeding activity compared with the same organs of the transgenic control plant. When a large-scale aphid population was monitored for 24 hours, there was a significant decrease in the number of aphids that colonized leaves of HrpN Ea -treated WT and PP2OETAt plants, respectively, compared with control plants.    Conclusions  The repression in phloem-feeding activities of  M. persicae  as a result of  AtPP2-A1  overexpression, and as a deterrent effect of HrpN Ea  treatment in WT Arabidopsis rather than the  atpp2-a1 /E/142 mutant suggest that  AtPP2-A1  plays a role in plant resistance to the insect, particularly at the phloem-feeding stage. The accompanied change of aphid population in leaf colonies suggests that the function of  AtPP2-A1  is related to colonization of the plant.

bmc plant biology

Harpin-induced expression and transgenic overexpression of the phloem protein gene AtPP2-A1 in Arabidopsis repress phloem feeding of the green peach aphid Myzus persicae