English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  In the last hundred years, the development of improved wheat cultivars has led to the replacement of landraces and traditional varieties by modern cultivars. This has resulted in a decline in the genetic diversity of agriculturally used wheat. However, the diversity lost in the elite material is somewhat preserved in crop gene banks. Therefore, the gene bank accessions provide the basis for genetic improvement of crops for specific traits and and represent rich sources of novel allelic variation.    Results  We have undertaken large scale molecular allele mining to isolate new alleles of the powdery mildew resistance gene  Pm3  from wheat gene bank accessions. The search for new  Pm3  alleles was carried out on a geographically diverse set of 733 wheat accessions originating from 20 countries.  Pm3  specific molecular tools as well as classical pathogenicity tests were used to characterize the accessions. Two new functional  Pm3  alleles were identified out of the eight newly cloned  Pm3  sequences. These new resistance alleles were isolated from accessions from China and Nepal. Thus, the repertoire of functional  Pm3  alleles now includes 17 genes, making it one of the largest allelic series of plant resistance genes. The combined information on resistant and susceptible  Pm3  sequences will allow to study molecular function and specificity of functional  Pm3  alleles.    Conclusions  This study demonstrates that molecular allele mining on geographically defined accessions is a useful strategy to rapidly characterize the diversity of gene bank accessions at a specific genetic locus of agronomical importance. The identified wheat accessions with new resistance specificities can be used for marker-assisted transfer of the  Pm3  alleles to modern wheat lines.

Wheat gene bank accessions as a source of new alleles of the powdery mildew resistance gene Pm3: a large scale allele mining project