English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  The yellow colour of pasta products is one of the main criteria used by consumers to assess pasta quality. This character is due to the presence of carotenoid pigments in semolina. During pasta processing, oxidative degradation of carotenoid pigments occurs mainly due to lipoxygenase (LOX). In durum wheat ( Triticum durum  Desf.), two  Lpx-1  genes have been identified on chromosome 4B,  Lpx-B1.1  and  Lpx-B1.2 , and evidences have been reported that the deletion of  Lpx-B1.1  is associated with a strong reduction in LOX activity in semolina. In the present study, we characterised the  Lpx-B1  gene family identified in a durum wheat germplasm collection and related the distribution and expression of the  Lpx-B1  genes and alleles to variations in LOX activity in the mature grains.    Results  In addition to the already known  Lpx-B1.1  and  Lpx-B1.2  genes, a new gene was identified,  Lpx-B1.3 , along with three different  Lpx-B1.1  alleles,  Lpx-B1.1a ,  Lpx-B1.1b  and the partially deleted  Lpx-B1.1c . Screening of the germplasm collection showed that all of the genotypes have one of the three  Lpx-B1.1  alleles, associated with either  Lpx-B1.2  or  Lpx-B1.3 , thus showing that in this collection the two genes are alternatives. Therefore, based on  Lpx-B1  distribution, three different haplotypes were distinguished: haplotype I, carrying  Lpx-B1.3  and the  Lpx-B1.1b  allele; haplotype II carrying  Lpx-B1.2  and the  Lpx-B1.1a  allele; and haplotype III carrying  Lpx-B1.2  and the  Lpx-B1.1c  allele. Determination of  Lpx-B1  transcript abundance and total LOX activity in mature grains revealed differences among these three haplotypes: haplotypes I, II and III showed high, intermediate and low levels, respectively, of functional  Lpx-B1  transcripts and enzymatic activity.    Conclusions  In this germplasm collection, the  Lpx-B1  gene family accounts for most of the total LOX activity in the mature grains. Information on these  Lpx-B1  haplotypes provides significant improvement for prediction of LOX-1 activity levels in mature grains, and will therefore help in breeding programmes aimed at selection of new durum wheat genotypes with higher carotenoid contents in their end products.

Insight into durum wheat Lpx-B1: a small gene family coding for the lipoxygenase responsible for carotenoid bleaching in mature grains