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Background  The interaction of plants with endophytic symbiotic fungi in the genus  Trichoderma  alters the plant proteome and transcriptome and results in enhanced plant growth and resistance to diseases. In a previous study, we identified the numerous chitinolytic enzyme families and individual enzymes in maize which are implicated in plant disease resistance and other plant responses.    Results  We examined the differential expression of the entire suite of chitinolytic enzymes in maize plants in the presence and absence of  T. harzianum . Expression of these enzymes revealed a band of chitinolytic enzyme activity that had greater mass than any known chitinase. This study reports the characterization of this large protein. It was found to be a heretofore undiscovered heterodimer between an exo- and an endo-enzyme, and the endo portion differed between plants colonized with  T. harzianum  and those grown in its absence and between shoots and roots. The heterodimeric enzymes from shoots in the presence and absence of  T. harzianum  were purified and characterized. The dimeric enzyme from  Trichoderma -inoculated plants had higher specific activity and greater ability to inhibit fungal growth than those from control plants. The activity of specific chitinolytic enzymes was higher in plants grown from  Trichoderma  treated seeds than in control plants.    Conclusions  This is the first report of a dimer between endo- and exochitinase. The endochitinase component of the dimer changed post  Trichoderma  inoculation. The dimer originating from  Trichoderma  inoculated plants had a higher antifungal activity than the comparable enzyme from control plants.

bmc plant biology

Differential expression of maize chitinases in the presence or absence of Trichoderma harzianum strain T22 and indications of a novel exo- endo-heterodimeric chitinase activity