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. The latter can be ascribed to an efficientamplification cycle whereby bilirubin, acting as antioxi-dant, is itself oxidized to biliverdin and then recycled backto bilirubin by NADPH-dependent biliverdin reductase.Bilitranslocase, a bilirubin-specific membrane carrier thatmediates cellular uptake of bilirubin, has recently beenfound in the vascular endothelium. However, the levels ofalbumin-free bilirubin in plasma and tissues are only 10to 50 nM. Therefore, the objective of this study was to elu-cidate the antioxidant activity of low concentrations ofbilirubin and the involvement of bilitranslocase-medi-ated plasma membrane transport in human endothelialcells.

Cellular antioxidant activity of bilirubin in the human endothelial cell line EA.hy 926 is mediated by bilitranslocase