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Background CB1 and CB2 cannabinoid receptors are G protein-coupled receptors which have been described to couple mainly to the Gi/o subfamily of G proteins. However, in some cell types and upon stimulation with certain cannabinoid agonists, activation of other G protein subtypes has also been observed. GPR55 is an orphan G protein-coupled receptor which has been suggested to be a novel member of the cannabinoid receptor family.

Characterization of different G protein coupling properties of CB1 and CB2 cannabinoid receptors and GPR55 receptor using BRET