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3,5-Di-t-butyl catechol (DTCAT) has been shown to release Ca2+ from rat skeletal muscle sarcoplasmic reticulum (SR) vesicles, which makes it a possible candidate for use as a substitute for halothane or caffeine in the in vitro contracture test (IVCT) for the assessment of susceptibility to malignant hyperthermia (MHS).

bmc pharmacology

3,5-Di-t-butyl catechol (DTCAT) as an activator of the human skeletal muscle ryanodine receptor Ca2+ channel and its evaluation as a test substance for the assessment of susceptibility to malignant hyperthermia