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Background The accurate reconstruction of neuronal morphology from image stacks obtained via microscopy is of significant importance to computational neuroscience. Firstly, it facilitates the validation of models of neuronal behaviour by allowing for comparisons between electrophysiological testing and simulation (through NEURON [1], for example). Secondly, through the use of appropriate morphometrics [2], significant differences between the shape of neurons in control and experimental conditions may be identified, thus lending insight into low-level components of neurological diseases. Also, there is a high level of interest in the reconstruction of larger scale networks within the nervous system and the identification of patterns of connectivity.

Improved automatic midline tracing of neurites with Neuromantic