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Background  The emerging concept is that microRNAs (miRNAs) play a central role in controlling stem cell self-renewal and fate determination by regulating the expression of stem cell regulators.  miR-125b , one of neuronal miRNAs, recently was found to be necessary for neural differentiation of neural stem/progenitor cells (NS/PCs). However, the other specific biological role of  miR-125b  in NS/PCs is little known. We used rat NS/PCs as a model system to study the role of  miR-125b  in governing the behavior of NS/PCs.    Results  We report here the transfection of exogenous  miR-125b  inhibited proliferation of NS/PCs but promoted differentiation and migration. Whereas anti-miR-125b had the opposite effect. Similar results were observed when  Nestin  was knocked down by siRNA. Subsequently, we demonstrated that  Nestin  was a direct functional target of  miR-125b .  MiR-125b  downregulates the expression of luciferase through  Nestin  3’untranslated region (3’-UTR), and the regulation was abolished by mutations in the  miR-125b  binding site.  MiR-125b  targeted the 3'-UTR of  Nestin  and reduced the abundance of  Nestin  at both mRNA and protein levels.    Conclusion  The results provided new insight into the function by which  miR-125b  modulates NS/PCs proliferation, differentiation and migration. The data also indicated the regulatory role of  miR-125b  in NS/PCs might through the suppression of  Nestin  expression.

MiR-125b orchestrates cell proliferation, differentiation and migration in neural stem/progenitor cells by targeting Nestin