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Abstract Background Exposure of <it>Leishmania </it>promastigotes to the temperature of their mammalian hosts results in the induction of a typical heat shock response. It has been suggested that heat shock proteins play an important role in parasite survival and differentiation. Results Here we report the studies on the expression of the heat shock protein 83 (<it>HSP83</it>) genes of <it>Leishmania infantum</it>. Confirming previous observations for other <it>Leishmania </it>species, we found that the <it>L. infantum HSP83 </it>transcripts also show a temperature-dependent accumulation that is controlled by a post-transcriptional mechanism involving sequences located in the 3'-untranslated region (3'-UTR). However, contrary to that described for <it>L. amazonensis</it>, the accumulation of the <it>HSP83 </it>transcripts in <it>L. infantum </it>is dependent on active protein synthesis. The translation of <it>HSP83 </it>transcripts is enhanced during heat shock and, as first described in <it>L. amazonensis</it>, we show that the 3'-UTR of the <it>L. infantum HSP83 </it>gene is essential for this translational control. Measurement of the steady-state levels of <it>HSP83 </it>transcripts along the promastigote-to-amastigote differentiation evidenced a specific profile of <it>HSP83 </it>RNAs: after an initial accumulation of <it>HSP83 </it>transcripts observed short after (2 h) incubation in the differentiation conditions, the amount of <it>HSP83 </it>RNA decreased to a steady-state level lower than in undifferentiated promastigotes. We show that this transient accumulation is linked to the presence of the 3'-UTR and flanking regions. Again, an 8-fold increase in translation of the <it>HSP83 </it>transcripts is observed short after the initiation of the axenic differentiation, but it is not sustained after 9 h. Conclusions This transient expression of <it>HSP83 </it>genes could be relevant for the differentiation of <it>Leishmania</it>, and the underlying regulatory mechanism may be part of the developmental program of this parasite.</p

bmc molecular biology

The expression of HSP83 genes in Leishmania infantum is affected by temperature and by stage-differentiation and is regulated at the levels of mRNA stability and translation.