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Abstract Background Termination of protein synthesis in eukaryotes involves at least two polypeptide release factors (eRFs) – eRF1 and eRF3. The highly conserved translation termination factor eRF1 in <it>Saccharomyces cerevisiae </it>is encoded by the essential gene <it>SUP45</it>. Results We have isolated five <it>sup45-n (n </it>from nonsense) mutations that cause nonsense substitutions in the following amino acid positions of eRF1: Y53 → UAA, E266 → UAA, L283 → UAA, L317 → UGA, E385 → UAA. We found that full-length eRF1 protein is present in all mutants, although in decreased amounts. All mutations are situated in a weak termination context. All these <it>sup45-n </it>mutations are viable in different genetic backgrounds, however their viability increases after growth in the absence of wild-type allele. Any of <it>sup45-n </it>mutations result in temperature sensitivity (37°C). Most of the <it>sup45-n </it>mutations lead to decreased spore viability and spores bearing <it>sup45-n </it>mutations are characterized by limited budding after germination leading to formation of microcolonies of 4–20 cells. Conclusions Nonsense mutations in the essential gene <it>SUP45 </it>can be isolated in the absence of tRNA nonsense suppressors.</p

Viable nonsense mutants for the essential gene SUP45 of Saccharomyces cerevisiae