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Background  RA (retinoic acid) signal pathway has been proved to be required for germ cell meiotic initiation in mammals, aves and amphibians. Stra8 (Stimulated by retinoic acid gene 8) is an important factor in RA signal pathway. However, the role of RA and Stra8 in germ cell meiotic initiation in teleosts is poorly characterized.    Results  In this study, the full length cDNA of  Stra8  was cloned from Southern catfish ( Silurus meridionalis ), and its spatio-temporal expression profiles were analyzed. The  Stra8  cDNA (1606 bp) includes 163 bp 5’-UTR (untranslated region), 456 bp 3’-UTR, and an ORF (open reading frame) of 987 bp, encoding a polypeptide of 328 aa. Phylogenetic analysis revealed its existence in some primitive teleosts, such as Siluriformes and Salmoniformes. Tissue distribution analysis by RT-PCR showed that  Stra8  is specifically expressed in gonads. By real-time PCR,  in situ  hybridization and immunohistochemistry, the highest expression level of  Stra8/ Stra8 was detected in 50 and 130 dah (day after hatching), the premeiotic stage of germ cells in XX and XY gonads, respectively.    Conclusions  Our results suggest that Stra8 might be involved in germ cell meiotic initiation in  S. meridionalis  as it did in tetrapods.

Characterization of Stra8 in Southern catfish (Silurus meridionalis): evidence for its role in meiotic initiation