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Background  Over the last decade, cholera outbreaks in parts of Kenya have become common. Although a number of recent studies describe the epidemiology of cholera in Kenya, there is paucity of information concerning the diversity and occurrence of mobile genetic elements in  Vibrio cholerae  strains implicated in these outbreaks. A total of 65  Vibrio cholerae  O1 El Tor serotype Inaba isolated between 1994 and 2007 from various outbreaks in Kenya were investigated for mobile genetic elements including integrons, transposons, the integrating conjugative elements (ICEs), conjugative plasmids and for their genotypic relatedness.    Results  All the strains were haemolytic on 5% sheep blood and positive for the  Vibrio cholerae  El Tor-specific haemolysin toxin gene ( hylA ) by PCR. They all contained  strB, sulII, floR  and the  dfrA1  genes encoding resistance to streptomycin, sulfamethoxazole, chloramphenicol and trimethoprim respectively. These genes, together with an ICE belonging to the SXT/R391 family were transferable to the rifampicin-resistant  E. coli  C600  en bloc . All the strains were negative for integron class 1, 2 and 3 and for transposase gene of transposon  Tn 7 but were positive for integron class 4 and the  trpM  gene of transposon  Tn 21. No plasmids were isolated from any of the 65 strains. All the strains were also positive for all  V. cholera  El Tor pathogenic genes except the NAG- specific heat-stable toxin ( st ) gene. None of the strains were positive for virulence genes associated with the  V. cholerae  classical biotype. All the strains were positive for El Tor-specific CTXphi bacteriophage  rstrR  repressor gene ( CTX  ET  Φ ) but negative for the Classical, Calcutta, and the Environmental repressor types. Pulse Field Gel Electrophoresis (PFGE) showed that regardless of the year of isolation, all the strains bearing the SXT element were clonally related.    Conclusions  This study demonstrates that the  V. cholerae  O1 strains carrying an SXT/R391-like element implicated in recent cholera outbreaks in Kenya has not changed significantly between 1994 and 2007 and are clonally related.

Molecular characterisation of Vibrio cholerae O1 strains carrying an SXT/R391-like element from cholera outbreaks in Kenya: 1994-2007