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Background  Soya and its derivatives represent nutritionally high quality food products whose major drawback is their high content of α-galacto-oligosaccharides. These are not digested in the small intestine due to the natural absence of tissular α-galactosidase in mammals. The passage of these carbohydrates to the large intestine makes them available for fermentation by gas-producing bacteria leading to intestinal flatulence. The aim of the work reported here was to assess the ability of α-galactosidase-producing lactobacilli to improve the digestibility of α-galacto-oligosaccharides  in situ .    Results  Gnotobiotic rats were orally fed with soy milk and placed in respiratory chambers designed to monitor fermentative gas excretion. The validity of the animal model was first checked using gnotobiotic rats monoassociated with a  Clostridium butyricum  hydrogen (H 2 )-producing strain. Ingestion of native soy milk by these rats caused significant H 2  emission while ingestion of α-galacto-oligosaccharide-free soy milk did not, thus validating the experimental system. When native soy milk was fermented using the α-galactosidase-producing  Lactobacillus fermentum  CRL722 strain, the resulting product failed to induce H 2  emission in rats thus validating the bacterial model. When  L. fermentum  CRL722 was coadministered with native soy milk, a significant reduction (50 %,  P  = 0.019) in H 2  emission was observed, showing that α-galactosidase from  L. fermentum  CRL722 remained active  in situ , in the gastrointestinal tract of rats monoassociated with  C. butyricum . In human-microbiota associated rats,  L. fermentum  CRL722 also induced a significant reduction of H 2  emission (70 %,  P  = 0.004).    Conclusion  These results strongly suggest that  L. fermentum  α-galactosidase is able to partially alleviate α-galactosidase deficiency in rats. This offers interesting perspectives in various applications in which lactic acid bacteria could be used as a vector for delivery of digestive enzymes in man and animals.

bmc microbiology

Ability of Lactobacillus fermentum to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides