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Background   Leptospira interrogans  is an important mammalian pathogen. Transmission from an environmental source requires adaptation to a range of new environmental conditions in the organs and tissues of the infected host. Several studies have shown that a shift in culture temperature from 28°C to 37°C, similar to that encountered during infection of a host from an environmental source, is associated with differential synthesis of several proteins of the outer membrane, periplasm and cytoplasm. The whole genome of the  Leptospira interrogans serogroup Icterohaemorrhagiae serovar lai type strain #56601  was sequenced in 2003 and microarrays were constructed to compare differential transcription of the whole genome at 37°C and 28°C.    Results  DNA microarray analyses were used to investigate the influence of temperature on global gene expression in  L. interrogans  grown to mid-exponential phase at 28°C and 37°C. Expression of 106 genes differed significantly at the two temperatures. The differentially expressed genes belonged to nine functional categories: Cell wall/membrane biogenesis genes, hemolysin genes, heat shock proteins genes, intracellular trafficking and secretion genes, two-component system and transcriptional regulator genes, information storage and processing genes, chemotaxis and flagellar genes, metabolism genes and genes with no known homologue. Real-time reverse transcription-PCR assays confirmed the microarray data.    Conclusion  Microarray analyses demonstrated that  L. interrogans  responds globally to temperature alteration. The data delineate the spectrum of temperature-regulated gene expression in an important human pathogen and provide many new insights into its pathogenesis.

Genome-wide transcriptional analysis of temperature shift in L. interrogans serovar lai strain 56601